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random primer oligonucleotides  (TaKaRa)


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    Structured Review

    TaKaRa random primer oligonucleotides
    Random Primer Oligonucleotides, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6514 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+oligonucleotide+primers/Random+Primer/pmc12912838-52-14-17
    Average 96 stars, based on 6514 article reviews
    random primer oligonucleotides - by Bioz Stars, 2026-09
    96/100 stars

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    Produced:

    Article Title: Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.
    Article Snippet: Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum.. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome.. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced.

    Polymerase Chain Reaction:

    Article Title: Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.
    Article Snippet: Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum.. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome.. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced.

    Article Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
    Article Snippet: The N-terminal amino-acid sequence ofα-amylase was determined with an ABI Procise 492 sequencer (Applied Biosystems, Foster City, CA, USA) using the samples electrically transferred to the polyvinylidenedifluoride membrane (Applied Biosystems) after SDS-PAGE. .. Total RNAwas extracted from the hepatopancreas of abalone by the guanidinium thiocyanate–phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturer's protocol. cDNA was synthesized from themRNAwith a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. cDNAs encoding α-amylase were amplified by PCR with degenerated primers synthesized on the basis of the N-terminal amino-acid sequence of theα-amylase and the internal amino-acid sequences conserved among α-amylases previously reported. .. For the amplification of cDNA, a successive reaction at 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 120 s was repeated 30 cycles in 20 μL of reaction mixture containing 50 mM KCl, 15 mM Tris–HCl (pH 8.1), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 2.5 mM MgCl2, 10 pmol primers, 20 ng hepatopancreas cDNA, and 0.5 U of TaKaRa Taq DNA polymerase.

    Plasmid Preparation:

    Article Title: Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.
    Article Snippet: Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum.. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome.. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced.

    Labeling:

    Article Title: Diversity of organophosphorus pesticide-degrading bacteria in a polluted soil and conservation of their organophosphorus hydrolase genes.
    Article Snippet: Seven methyl parathion-degrading bacteria were isolated from a long-term methyl parathion contaminated soil and were found to belong to the genera Pseudaminobacter, Achromobacter, Brucella, and Ochrobactrum.. Southern blot analysis using an mpd gene probe revealed that their hydrolase genes were similar to the mpd gene from Plesiomonas sp. strain M6 and were all located on the chromosome.. Gene libraries were constructed from genomic DNA of each of the 7 organophosphorus pesticide-degrading bacteria, and their mpd genes were cloned and sequenced.

    Reverse Transcription:

    Article Title: Anti-inflammatory effects of madecassic acid via the suppression of NF-kappaB pathway in LPS-induced RAW 264.7 macrophage cells.
    Article Snippet: We have investigated the anti-inflammatory effects of madecassic acid and madecassoside isolated from Centella asiatica (Umbelliferae) on lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells.. Both madecassic acid and madecassoside inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6.. However, madecassic acid more potently suppressed these inflammatory mediators than did madecassoside.

    Article Title: High FGF18 expression levels predict poor prognosis in endometrial carcinoma patients and promote tumor growth and metastasis
    Article Snippet: .. Fragmented mRNA was reverse transcribed into first-strand cDNA using M-MuLV reverse transcriptase and random oligonucleotide primers (Takara Bio, Dalian, China). ..

    Article Title: High FGF18 expression levels predict poor prognosis in endometrial carcinoma patients and promote tumor growth and metastasis.
    Article Snippet: .. Fragmented mRNA was reverse transcribed into first-strand cDNA using M-MuLV reverse transcriptase and random oligonucleotide primers (Takara Bio, Dalian, China). ..

    Synthesized:

    Article Title: Characterization of an α-glucosidase, HdAgl, from the digestive fluid of Haliotis discus hannai.
    Article Snippet: .. Total RNA was extracted from the hepatopancreas of abalone by the guanidinium thiocyanate-phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturers' protocol. cDNA was synthesized from the mRNA with a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. ..

    Article Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
    Article Snippet: The N-terminal amino-acid sequence ofα-amylase was determined with an ABI Procise 492 sequencer (Applied Biosystems, Foster City, CA, USA) using the samples electrically transferred to the polyvinylidenedifluoride membrane (Applied Biosystems) after SDS-PAGE. .. Total RNAwas extracted from the hepatopancreas of abalone by the guanidinium thiocyanate–phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturer's protocol. cDNA was synthesized from themRNAwith a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. cDNAs encoding α-amylase were amplified by PCR with degenerated primers synthesized on the basis of the N-terminal amino-acid sequence of theα-amylase and the internal amino-acid sequences conserved among α-amylases previously reported. .. For the amplification of cDNA, a successive reaction at 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 120 s was repeated 30 cycles in 20 μL of reaction mixture containing 50 mM KCl, 15 mM Tris–HCl (pH 8.1), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 2.5 mM MgCl2, 10 pmol primers, 20 ng hepatopancreas cDNA, and 0.5 U of TaKaRa Taq DNA polymerase.

    cDNA Synthesis:

    Article Title: Characterization of an α-glucosidase, HdAgl, from the digestive fluid of Haliotis discus hannai.
    Article Snippet: .. Total RNA was extracted from the hepatopancreas of abalone by the guanidinium thiocyanate-phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturers' protocol. cDNA was synthesized from the mRNA with a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. ..

    Article Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
    Article Snippet: The N-terminal amino-acid sequence ofα-amylase was determined with an ABI Procise 492 sequencer (Applied Biosystems, Foster City, CA, USA) using the samples electrically transferred to the polyvinylidenedifluoride membrane (Applied Biosystems) after SDS-PAGE. .. Total RNAwas extracted from the hepatopancreas of abalone by the guanidinium thiocyanate–phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturer's protocol. cDNA was synthesized from themRNAwith a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. cDNAs encoding α-amylase were amplified by PCR with degenerated primers synthesized on the basis of the N-terminal amino-acid sequence of theα-amylase and the internal amino-acid sequences conserved among α-amylases previously reported. .. For the amplification of cDNA, a successive reaction at 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 120 s was repeated 30 cycles in 20 μL of reaction mixture containing 50 mM KCl, 15 mM Tris–HCl (pH 8.1), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 2.5 mM MgCl2, 10 pmol primers, 20 ng hepatopancreas cDNA, and 0.5 U of TaKaRa Taq DNA polymerase.

    Amplification:

    Article Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
    Article Snippet: The N-terminal amino-acid sequence ofα-amylase was determined with an ABI Procise 492 sequencer (Applied Biosystems, Foster City, CA, USA) using the samples electrically transferred to the polyvinylidenedifluoride membrane (Applied Biosystems) after SDS-PAGE. .. Total RNAwas extracted from the hepatopancreas of abalone by the guanidinium thiocyanate–phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturer's protocol. cDNA was synthesized from themRNAwith a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. cDNAs encoding α-amylase were amplified by PCR with degenerated primers synthesized on the basis of the N-terminal amino-acid sequence of theα-amylase and the internal amino-acid sequences conserved among α-amylases previously reported. .. For the amplification of cDNA, a successive reaction at 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 120 s was repeated 30 cycles in 20 μL of reaction mixture containing 50 mM KCl, 15 mM Tris–HCl (pH 8.1), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 2.5 mM MgCl2, 10 pmol primers, 20 ng hepatopancreas cDNA, and 0.5 U of TaKaRa Taq DNA polymerase.

    Sequencing:

    Article Title: Enzymatic properties and primary structures of two α-amylase isozymes from the Pacific abalone Haliotis discus hannai.
    Article Snippet: The N-terminal amino-acid sequence ofα-amylase was determined with an ABI Procise 492 sequencer (Applied Biosystems, Foster City, CA, USA) using the samples electrically transferred to the polyvinylidenedifluoride membrane (Applied Biosystems) after SDS-PAGE. .. Total RNAwas extracted from the hepatopancreas of abalone by the guanidinium thiocyanate–phenol method (Chomczynski and Sacchi, 1987). mRNA was selected from the total RNA with an Oligo-dT(30) kit (TaKaRa) according to the manufacturer's protocol. cDNA was synthesized from themRNAwith a cDNA synthesis kit (TaKaRa) using random oligonucleotide primers. cDNAs encoding α-amylase were amplified by PCR with degenerated primers synthesized on the basis of the N-terminal amino-acid sequence of theα-amylase and the internal amino-acid sequences conserved among α-amylases previously reported. .. For the amplification of cDNA, a successive reaction at 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 120 s was repeated 30 cycles in 20 μL of reaction mixture containing 50 mM KCl, 15 mM Tris–HCl (pH 8.1), 0.2 mM each of dATP, dTTP, dGTP, and dCTP, 2.5 mM MgCl2, 10 pmol primers, 20 ng hepatopancreas cDNA, and 0.5 U of TaKaRa Taq DNA polymerase.



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